被動免疫和保護測試的文章翻譯

The experimental protocols were reviewed and approved by theHokkaido University Animal Care and Use Committee (08-0234).Six-week-old female BALB/c mice were passively immunized byintraperitoneal injection with 200 mg of purified MAb S139/1 orZGP12/1.1 (IgG2a) [18] in 0.5 ml of PBS. Twenty four hoursbefore or after immunization

mice were challenged intranasallywith 50 ml of 10650% mouse infectious dose of Aichi (H3) orWSN (H1) under anesthesia with isoflurane. Three days after thechallenge

mice were euthanized to obtain the lung tissue samples.The lung homogenates (10% w/v) prepared in MEM weredisrupted and centrifuged at 3

0006g for 10 min

and then thesupernatants were examined for virus infectivity. Virus titers weremeasured by a plaque assay using MDCK cells.
實驗協議進行審查和批准 北海道大學動物護理和使用委員會（08-0234）。

六週齡雌性BALB / c小鼠的被動免疫 腹腔注射200毫克的純化抗體S139 / 1或 ZGP12/1.1（IgG2a的）[18]在0.5毫升的PBS。

24個小時 免疫之前或之後

小鼠鼻腔挑戰 50毫升10650％小鼠感染劑量的愛知縣（H3的）或 無線傳感器網絡（上半年）異氟醚麻醉下。

三天後

挑戰

小鼠安樂死

以獲取肺組織樣本。

肺組織勻漿（10％瓦特/五）編寫的培養基是 干擾和離心3

0006克10分鐘

然後 上清審查了病毒的傳染性。

病毒滴度 衡量一空斑測定MDCK細胞使用。

參考資料 我 Google翻譯 :D

Hokkaido University Animal,intraperitoneal injection,experimental protocols,北海道大學動物護理和使用委員會,免疫,PBS,challenged intranasally,無線傳感器網絡,plaque assay,purified MAb

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